Hyaluronidases hyals are a family of hydrolytic enzymes that degrade hyaluronic acid. Download fulltext pdf download fulltext pdf hyaluronidase. There are three types of uses approved by the us fda. Hyaluronidase hase was dominantly expressed at invasive malignant glioma cells, while it was not expressed at normal brain tissues, and is known to be responsible for brain metastases. Each vial contains 150 usp units of hyaluronidase per ml calcium. For experimental use the lyophilized enzyme was dissolved in acetate buffer of ph 6. The hyaluronidase effects in this context persist for approximately 48 hours. Hyaluronic acid is a mucopolysacharide that is part of the ground substance of the dermis so the enzyme hyaluronidase is part of the bodies homeostatic mechanism. Injected hyaluronidase reduces restylanemediated cutaneous. By 4 to 7 days after hyaluronidase injection, skin scores were at 20% of baseline p pdf link pdf citation sami albeiroti, katayoun ayasoufi, david r. Hyaluronidase is used to improve the spread of local. Clinical applications of hyaluronidase springerlink.
Hyaluronidase cleaves endonacetlyhexosaminic bonds in hyaluronic acid and chondroitin sulfate a and c to tetrasaccharide residues, as hyaluronic acid and chondroitin sulfate are often found in. Group a streptococcus gas depends on a hyaluronic acid ha capsule to evade phagocytosis and to interact with epithelial cells. Us2808362a us285792a us28579252a us2808362a us 2808362 a us2808362 a us 2808362a us 285792 a us285792 a us 285792a us 28579252 a us28579252 a us 28579252a us 2808362 a us2808362 a us 2808362a authority us united states prior art keywords hyaluronidase extract tissue water aqueous extract prior art date 19520502 legal status the legal status is an assumption and is. The exact chemical structure of this enzyme is unknown.
They also expressed the view that the denudation of the egg. Hyaluronidase is an enzyme that depolymerizes the mucopolysaccharide hyaluronic acid, which is a component of the mucoprotein ground substance or tissue cement and thus increases membrane permeability. The hashnfs were noncytotoxic to vaginal vk2e6e7 and end1e6e7. The preparation method of the recombinant human hyaluronidase comprises the steps of integrating a human hyaluronidase gene into pichia pastoris engineering bacteria, fermenting and purifying, so that a large number of highactivity and highpurity recombinant human hyaluronidase can be obtained. Hylenex recombinant hyaluronidase human injection is an. This enzyme is able to promote the drugs diffusion into the extracellular matrix and to increase blood vessel permeability. This enzyme is active at an acidic ph and is the major hyaluronidase in plasma. Its use assists in the dispersal of tissue fluids or injected drugs. Download center enzyme introduction enzyme families nanozyme enzyme assays protocols. The enzyme nature of this substance was, however, denied 2.
Cosmetically hyaluronic acid is injected into people to improve wrinkles. Standardized in vitro analysis of the degradability of. Hyaluronidase is an enzyme that degrades hyaluronic acid. Hyaluronidase is a term applied to a group of very dissimilar enzymes 1, 2, 3 that degrade hyaluronan ha, hyaluronic acid, a highmolecularweight glycosaminoglycan of the extracellular matrix. Hyaluronidase catalyzes the random hydrolysis of 1,4linkages between 2acetamido2deoxybdglucose and dglucose residues in hyaluronate.
The gene is one of several related genes in a region of chromosome 3p21. Mutations in this gene are associated with mucopolysaccharidosis type ix, or hyaluronidase deficiency. Role of hyaluronidase in the treatment of complications from. Hyaluronidase activity and hyaluronidase inhibitors. Hyaluronidases are employed for medical use to increase tissue absorption of several drugs. Hyaluronidase cleaves endonacetlyhexosaminic bonds in hyaluronic acid and chondroitin sulfate a and c to tetrasaccharide residues, as hyaluronic acid and chondroitin sulfate are often found in connective tissues. These help speed up the natural breakdown of hyaluronic acid through hydrolysis. Download hyaluronidase consent form have you had a client who is in need of a reversal following treatment with dermal fillers made of hyaluronic acid. Background hyaluronidase are a group of enzymes that degrade hayluronic acid ha. Hydase hyaluronidase injection is a preparation of purified bovine testicular hyaluronidase, a protein enzyme. Pdf link pdf citation sami albeiroti, katayoun ayasoufi, david r. Rather, perivascular hyaluronidase will permeate the vascular walls to decomplex intravascular ha.
Dailymed vitrase hyaluronidase, ovine injection, solution. Materials and methods hyaluronidase was extracted from fresh bull testes by essentially the same method as that described by hahn 7. The enzyme is sorted to the outer surface of the cell membrane of xkh1 expressing oocytes. Mutations in this gene are associated with mucopolysaccharidosis. Jan 04, 2019 hyaluronidase is a naturally occurring enzyme thats injected by a dermatologist or plastic surgeon into the skin to break down hyaluronic acidbased fillers, such as juvederm, restylane, or belotero. Before proceeding with the reverse protocol, it is vital that you gain consent from your patient. It was observed that in the absence of haase, the drug release from nfs followed the peppas kinetic model whereas, in the presence of haase, nfs followed weibull model. Therefore, the enzyme has been used as a local adjuvant to increase the diffusion capacity of local. Efficacy of hyaluronidase in reducing increases in. Hyaluronidase enzyme solution immediately before use prepare a solution containing 3 5 units per ml in cold reagent d.
From clinical applications to molecular and cellular mechanisms article pdf available december 2016 with 1,763 reads. Hyaluronidase hyaluronidase for hyaluronic acid hydrolysis. Feb, 2016 hyaluronidase should not only be administered directly at the site of hafiller injection, but also along the course of the obstructed artery. Hyaluronidase hylase dessau is a hyaluronic acidmetabolizing enzyme, which has been shown to enhance drug diffusion and thereby increasing the efficacy. Some of these enzymes have the ability to degrade additional glycosaminoglycans, albeit at a slower rate. A comparison of average scores of salineinjected sites vs hyaluronidaseinjected sites revealed a statistically significant difference. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. Red willow road, wrexham, clwydd is an enzyme which depolymerises hyaluronic acid. It is a purified preparation of the enzyme recombinant human hyaluronidase and is produced by genetically engineered chinese hamster ovary cho cells containing a dna plasmid encoding for a soluble fragment of human hyaluronidase ph20.
We inactivated the gene encoding extracellular hyaluronidase, hyla. Pdf over the past 60 years, hyaluronidase has been successfully utilized in ophthalmic surgery and is now being. Recombinant human hyaluronidase ph20 belongs to a family of enzymes that catalyze the degradation of hyaluronic acid and can degrade hyaluronic acid in the extracellular matrix and promote the diffusion of extracellular substances. Nov 16, 2015 the reconstitution of the dermal barrier removed by intradermal injection of hyaluronidase 20, 2, 0. Top 21 on hyaluronidase healthtap healthtap healthtap. Also known as hyaluronan is composed of alternating residues of. Hyaluronidase, any of a group of enzymes that catalyze the hydrolysis chemical decomposition involving the elements of water of certain complex carbohydrates such as hyaluronic acid and chondroitin sulfates.
Furthermore, it is known that hyaluronic acid is readily hydrolyzed by the enzyme hyaluronidase. This product is derived from sheep or cows to create an enzyme antedote for ha based fillers. Importantly, direct intraarterial injection of hyaluronidase is not necessary or possible. Afterwards, mcclean and rowlands 4 and fekete and duranreynals 5 produced evidence to show that the enzyme is probably hyaluronidase. If too much is injected it can be removed by injecting hyaluronidase.
Hyaluronidase definition of hyaluronidase by medical. By 4 to 7 days after hyaluronidase injection, skin scores were at 20% of baseline p hyaluronidase enzyme. Hyaluronidase dramatically reduced the size of the augmentation created by injected restylane in all of our subjects. The study of histological changes of the arterial vascular. Hyaluronidase is an enzyme that depolymerizes the mucopolysaccharide hyaluronic. Paradoxically, gas also produces hyaluronidase hyl, an enzyme that cleaves ha. The offlabel use of hyaluronidase is regarded as gold standard for the management of hafillerassociated complications.
Have you had a client who is in need of a reversal following treatment with dermal fillers made of hyaluronic acid. Hyaluronidase is widely used in many fields, such as plastic surgery, ophthalmology, internal medicine, oncology treatment, dermatology, and gynecology. Jaques national institute for medical research, mill hill, london received 25 june 1952different workers have at various times sought to assay seven different enzyme preparations by thecorrelate the in vivo and in vitro activities of more. Hyaluronidase is a hyaluronic acid ha metabolizing enzyme, which is approved as an adjuvant for infiltration anesthesia. The objective of this study is to evaluate histological changes of postmortem arteries after intravascular injection of hyaluronidase enzyme.
The hyaluronidase enzyme activity has been assayed by several methods. A 600nm standard a 600nm standard a 600nm blank hyaluronidase units from std curvedf unitsml enzyme enzyme aliquot df dilution factor enzyme aliquot volume in milliliters of enzyme used in assay unitsml enzyme. The enzymes have been found in insects, leeches, snake venom, mammalian tissues testis. Hyaluronidase should not only be administered directly at the site of hafiller injection, but also along the course of the obstructed artery. Hyaluronidases are a family of five human enzymes that have been differentially implicated in the progression of many solid tumor types, both clinically and in functional studies. Hyaluronidase is glycosylated and contains 5% mannose and 2.
Aug 20, 2018 hyaluronidase is a hyaluronic acid ha metabolizing enzyme, which is approved as an adjuvant for infiltration anesthesia. Hyaluronidase human recombinant was approved for use in the united states in december 2005. Left alone, a hyaluronic acidbased filler would eventually be broken down by your bodys own enzymes, but that process can take up to. Borders and raftery 1968 reported a molecular weight of 61,000. Download hyaluronidase consent form dr tim pearce, elearning. Here, we give an overview over the molecular and cellular mode of action of hyaluronidase and the hyaluronic acid metabolism, as well as over current and potential future clinical applications of hyaluronidase.
Hyaluronidase definition of hyaluronidase by medical dictionary. Official label printer friendly view all sections close all sections. A common assumption is that hyl digests structurally identical ha in human tissue to promote bacterial spread. Pdf identification and characterization of a bacterial. Hydase hyaluronidase injection is supplied as sterile, colorless, odorless, ready for use solution. In search for xenopus laevis hyaluronidase genes, a cdna encoding a putative ph20like. Ott from the department of chemistry and physics, army medical department research and graduate school, washington received for publication, septenlber 26,1947 a variety of methods have been devised for the assay of the enzyme hyaluronidase. Correlation between biological assay and other methods of assay by j. Antihyaluronidase sera, prepared against bovine hyaluronidase, crossreacted with both the treponemal hyaluronidase and testicular hyaluronidase, suggesting the treponemal hyaluronidase is similar to the hyaluronate4glycanohydrolase type enzymes and may not be a hyaluronate lyase. Xenopus kidney hyaluronidase1 xkh1, a novel type of membrane. Methods this was an ex vivo experiment including arterial specimens from four cadavers which recently deceased within 24 hours.
It also hydrolyses 1,4betadglycosidic linkages between nacetylgalactosamine or nacetylgalactosamine sulfate and glucuronic acid in chondroitin. Hyaluronidase is an enzyme that depolymerizes the mucopolysaccharide hyaluronic acid, which is a component of the mucoprotein ground substance or tissue cement and thus increases membrane permeability, reduces viscosity, and makes tissues more readily permeable 68 r. Characteristics of hyaluronidase from bovine testes. Cn104745553a recombinant human hyaluronidase, preparation. Adjunctive use of hyaluronidase in local anaesthesia journal of. The reason for this manifestation would appear to be a general defect in the cement substance of the skin.
We tested different concentrations of hyaluronidase 78 versus 39 iuml enzyme, 78 versus 10 iuml enzyme, 39 versus 10 iuml enzyme. It also hydrolyses 1,4betadglycosidic linkages between nacetylgalactosamine or nacetylgalactosamine sulfate and glucuronic acid in chondroitin sulfates a and c. This option is extremely useful in the event that a patient dislikes their results from restylane, juvederm, etc. Hyaluronidase h3884 product information sheet author. Left alone, a hyaluronic acidbased filler would eventually be broken down by your bodys own enzymes, but that process can take up to 18 months, depending on your metabolism. Page 2 of 3 enzymatic assay of hyaluronidase1 ec 3. Vitrase hyaluronidase injection is supplied as a sterile, nonpreserved, clear, colorless. Not for use in diagnostic or therapeutic procedures. Effects of different hyaluronidase concentrations and. Hyaluronidase from bovine testes is a tetramer of 4 equal subunits with a molecular mass of 14 kda each.
Objective to evaluate the efficacy of hyaluronidase in preventing increases in intraocular pressure related to injections of hyaluronancontaining viscoelastic substances methods twentyfive white rabbits were divided into 5 groups. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronidases of grampositive bacteria fems microbiology. Yet, up to date there are only few studies that have systematically assessed the degradability of different hafillers by hyaluronidase. Hyaluronidases are a family of injectable enzymes that act as dispersion agents. Further applications in the fields of surgery, aesthetic medicine, immunology, oncology, and many others can be expected for years to come. Role of hyaluronidase in subcutaneous spread and growth of. Ovine is a preparation of purified ovine testicular hyaluronidase, a protein enzyme. The enzyme is widely distributed in animal tissues but is found in great concentrations in the bovine and ovine testes. Hyaluronidase also known as vitrase is a medication that can be used to dissolve hyaluronic acid ha based dermal fillers such as restylane, perlane, juvederm, and more.
The reconstitution of the dermal barrier removed by intradermal injection of hyaluronidase over a range of 0. The highly viscous mucopolysaccharide hyaluronic acid has been shown to be a constituent of the cement substance of normal skin. Anti hyaluronidase sera, prepared against bovine hyaluronidase, crossreacted with both the treponemal hyaluronidase and testicular hyaluronidase, suggesting the treponemal hyaluronidase is similar to the hyaluronate4glycanohydrolase type enzymes and may not be a hyaluronate lyase. Pdf hyaluronidase activity and hyaluronidase inhibitors. The hyaluronidase enzyme hydrolyzes hyaluronan hyaluronic acid, a glycosaminoglycan found. The enzyme is considered a spreading factor as it decomplexes hyaluronic acid also called hyaluronan, ha, an essential component of the extracellular matrix ecm. A comparison of average scores of salineinjected sites vs hyaluronidase injected sites revealed a statistically significant difference.